When Yang Rui chose the correct degree, the correct DNA length, and the correct tracer, it would not be difficult to achieve results.

In fact, most graduate students work in their work and use known experimental methods to make results, which is the laboratory’s requirements for scientific research dogs.

Three years of master's degree, the first year is often mainly theoretical study and laboratory practice. At this time, if they do a middle school experiment, they may not be able to produce results.

But in the second year, when graduate students began to conduct experiments frequently, their speed and success rate of experiments were greatly improved.

If an experiment like the first generation PCR is not qualified to be called a scientific research dog if a student in the second generation cannot master it skillfully. At best, he is a wild dog, wandering between the laboratory and the laboratory corridor.

Of course, Yang Rui himself has never been exposed to the first-generation PCR. When domestic students come into contact with PCR, they are at least the second-generation PCR. They are extremely simple and common instruments, and their size is similar to those of microwave ovens. Open the lid, put the sample, press the button to select, and wait for the oven to be released.

Compared with the second-generation PCR, the difficulty of the first-generation PCR is equivalent to that of the oven. Yang Rui has been familiar with it after doing so many experiments. This time, he has seriously pretreated the raw materials, carefully added tracers to the raw materials, and carefully adjusted the temperature and time.

Then, Yang Rui seriously wrote "PCR128" in the experimental book.

However, everyone in the laboratory was serious and serious, Yang Rui attracted the ridicule of Wei Zhenxue.

"Yang Rui, even if you use official script to record it, it's useless. You have to do experiments, right? Oligonucleotides don't listen to you." Wei Zhenxue came to Yang Rui. His workload is not large now, and doing experiments is purely a personal hobby. He has published several papers and is very relaxed.

Yang Rui was in a good mood and said with a smile: "How do you know that oligonucleotides don't listen to me?"

Wei Zhenxue was inexplicably surprised and suddenly became very whispered: "Can you let oligonucleotides listen to you?"

After saying that, he looked back alertly and said, "What is your Qigong name? Molecular Qigong?"

Yang Rui was stunned: "How could you think of Qigong?"

"Why can't you think of Qigong? Look at you, you can't do it every day. Why do you do it so seriously today? You must have to do it. Otherwise..." Wei Zhenxue dragged his long voice, his eyes slanted, and said, "Why did you find a master to ask for advice, right?"

Yang Rui's expression seemed to be stared at by a dog, and he looked at Wei Zhenxue with a rabies-proof look.

Wei Zhenxue thought he saw Yang Rui through and smiled and said, "That's right, I guessed it through, you wait, I guess who did the method for you, Master Hu? He started a lecture at the Temple of Heaven recently, but he is very busy... Is that Master Zhu? Is Master Zhu from Dongcheng?"

"Old Wei!" Yang Rui had to interrupt him and looked at Wei Zhenxue with the boss's eyes: "You are quite idle lately and have learned so much."

"Live until you are old and learn until you are old." Wei Zhenxue smiled proudly.

"Learn Qigong and use Qigong for scientific research?"

"Oh, I'm also testing, hahaha..."

"Well, this is good. In the future, you should not use scientific research instruments in the laboratory, such as microscopes, and so on. Just measure Qigong. Save some instrument resources and allow everyone to be more abundant. You just think it's taking advantage of your remaining energy."

When Wei Zhenxue heard that he was not allowed to use instruments, he became anxious and said, "What are you using the residual heat? I am not old yet. I am just too young, so I haven't learned Qigong well. I haven't learned Qigong yet. Yang Rui, I still have to use instruments, but Qigong cannot be used."

"Is Qigong not good?"

"I can't use Qigong now."

"Is Qigong not good?"

"Hey, you're right, Qigong is not good!" Wei Zhenxue adheres to Chinese belief science and absolutely only chooses the advantageous ones.

Yang Rui damaged Wei Zhen’s words and felt that he was activate blood circulation and removed blood stasis, and his whole body felt relieved.

There must be someone in the laboratory to be scolded to be harmonious.

After rushing Wei Zhenxue to the side, Yang Rui began to simplify the system "PCR-128".

Compared with experiments with numbers before 50, the reaction volume of PCR-128 has also been reduced, and the reaction concentration has been further improved.

Yang Rui carefully recorded all the experimental preparations and experimental materials, and then stared at the water bath pot in a daze.

At this point, Yang Rui's experiment is slightly different from the original PCR.

Because the original PCR does not have heat-resistant polymerase, it will take a few seconds to come in and out of the water bath. When Muris was doing this experiment, he was like the boss of Malatang. He took a spoon on the mesh and put it in a while...

With heat-resistant polymerase, this step can be eliminated, and Yang Rui can be a little easier.

He calculated the time, put forward the things, and started running electrophoresis.

The purpose of running electrophoresis is to see the gel of radiotracer.

The gel is like jelly, and the DNA gathered by electrophoresis will form bands on the gel.

Getting the strips proves that there are products inside. If the strips are not obtained, the gel is still as clean as fresh jelly, then there is no product, which means the experiment fails.

Wei Zhenxue stood behind Yang Rui quietly, bent his knees slightly, stretched his hands flat, and his face was as red as constipation.

Yang Rui's attention was all focused on the gel, and he didn't know that anyone was watching behind him.

Yang Rui quietly waited for the electrophoresis to be completed, slowly pulled out the gel, and then placed it under the light of the experimental table to observe the gel carefully...

"Oh my God... I succeeded, I made a strip!" Wei Zhenxue suddenly shouted like a pig, his palm facing him with an expression of incredibility.

Yang Rui was so scared that he almost jumped up. When he turned around, he saw that it was Wei Zhenxue. He couldn't help but scolded: "What's your name!"

"I just helped you do your best, but you made strips. Look, you see, the color of the strips is a little lighter, but I made strips. What I did, what I did, what I did!" Wei Zhenxue kept pointing to himself.

"Have you really made a strip?" Huang Mao pushed Wei Zhenxue away, looked down at the gel, and said, "So, your more than 100 experiments have been done."

Not only Huang Mao, but everyone nodded with lingering fear.

Repeating more than a hundred experiments is a cruel behavior. Researchers with weaker psychological tolerance will collapse. Seeing that Yang Rui finally made something, everyone breathed a sigh of relief.

Yang Rui also breathed a sigh of relief.

Although the correct experimental method was used, there were still some results that could not be obtained when looking at the experimental manual.

"Look, I really made it and copied the same DNA. Is that what it means?" Li Wenqiang got closer and looked at the gel carefully.

"I just made my efforts." Wei Zhenxue also got closer.

Li Wenqiang gently pushed him away and said, "Don't make trouble."

"I've been practicing for half a year." Wei Zhenxue asked Zhang Xuetong, who came to join in the fun again.

"Be good." Zhang Xuetong patted Wei Zhenxue, turned a corner, and went to see the gel made by Yang Rui.

"There are indeed bands, which can be seen and done." Li Wenqiang nodded as he said, his expression did not change much from usual. After all, in his opinion, this should be a good paper, but it only ends with this.

Yang Rui re-checked the experiment. After confirming it, he called Wang Lei over without hesitation and said, "Do call Li Zhangzhen and ask him to find a patent lawyer as soon as possible! Please be a lawyer with high level, don't be afraid of spending money, it's best to be a large law firm."

"What lawyer?" Comrade Wang Lei, the new professional experiment, is not clear about these words.

"Patent lawyer, if you can't understand, call me first, and then call me." Yang Rui said again: "I'll do another repeated experiment. Zhou Yuwen, go and take out the camera in the cabinet and shoot it from beginning to end. Don't miss any details. Use the video recording opportunity. Be sure to pay attention when the tape is about to be finished and you can notify me in advance."

There is no need to worry about leaking secrets for PCR. This technology is priceless because it is easy to use and widely spread.

Compared to confidentiality, Yang Rui cares more about patents and honors.

Between the two, Yang Rui must first determine the honor. Video recording is the most common method used in later experiments, which is very convenient, simple and undoubtedly determine the ownership of the results.

There was no digital camera in 1984, but Yang Rui didn't care about wasting video tapes.

Huang Mao knew that Yang Rui was often targeted, so he thought about it for a while but couldn't figure it out.

The new Li Wentong and others felt that Yang Rui was making a big fuss, but the advantage of a private laboratory is that the boss is full of authority. Yang Rui uses his own money to do things, and others have nothing to say.
Chapter completed!